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rat anti mouse monoclonal f4 80  (Bio-Rad)


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    Bio-Rad rat anti mouse monoclonal f4 80
    Rat Anti Mouse Monoclonal F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 5687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+f4/Rat+anti+Mouse+F4%2F80/10__22203_slash_ecm__v033a11-129-8-16
    Average 96 stars, based on 5687 article reviews
    rat anti mouse monoclonal f4 80 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: CD74 Promotes Cyst Growth and Renal Fibrosis in Autosomal Dominant Polycystic Kidney Disease
    Article Snippet: .. After antigen retrieval, tissue sections were incubated with anti-F4/80 primary antibody (1:100, MCA497, BIO-RAD, Hercules, CA, USA), followed by incubation with Fluro-488 anti-rat IgG secondary antibody. .. Then the tissue sections were mounted in Prolong Gold Anti-fade reagent with DAPI (Invitrogen, Waltham, MA, USA).

    Article Title: Deletion of ABIN1-LIR motifs impairs hepatic lipid homeostasis and mitophagy via AMPK-TFEB axis in mice.
    Article Snippet: .. The slides are then blocked with 1X 300 blocking solution (Roti block, Roth, A151.1) for 30 min and incubated at 4 °C overnight with anti-301 neutrophil 7/4 antibody (Ly-6B.2, 1:500, Abcam, Cat# ab53457, RRID AB_881409) and anti-F4/80 302 (1:200, Bio Rad, Cat# MCA497G, RRID AB_872005). .. Detection was performed using Simple Stain Rat 303 MAX PO (R) (NICHIREI BIOSCIENCES INC., 414181F) followed by DAB substrate (VECTOR Laboratories, SK-304 4100) for chromogenic development.

    Article Title: Intestinal Effects of Filtered Alkalinized Water in Lean and Obese Zucker Rats
    Article Snippet: Tissue fixation and staining were performed using the Vectastain ABC kit and the DAB peroxidase substrate kit (both from Vector Laboratories, Burlingame, CA, USA) following the manufacturer’s instructions. .. Samples were incubated with anti-F4/80 primary antibody (MCA497; AbD Serotec, Oxford, UK) and then with anti-rat secondary antibody (NA935 GE Healthcare, Boston, MA, USA) linked to alkaline phosphatase (AP), exposed to the AP substrate and, once staining developed, mounted. .. Images were acquired with a Nikon E90i microscope equipped with a DS-Fi1 camera (Nikon, Tokyo, Japan).

    Blocking Assay:

    Article Title: Deletion of ABIN1-LIR motifs impairs hepatic lipid homeostasis and mitophagy via AMPK-TFEB axis in mice.
    Article Snippet: .. The slides are then blocked with 1X 300 blocking solution (Roti block, Roth, A151.1) for 30 min and incubated at 4 °C overnight with anti-301 neutrophil 7/4 antibody (Ly-6B.2, 1:500, Abcam, Cat# ab53457, RRID AB_881409) and anti-F4/80 302 (1:200, Bio Rad, Cat# MCA497G, RRID AB_872005). .. Detection was performed using Simple Stain Rat 303 MAX PO (R) (NICHIREI BIOSCIENCES INC., 414181F) followed by DAB substrate (VECTOR Laboratories, SK-304 4100) for chromogenic development.

    Staining:

    Article Title: Intestinal Effects of Filtered Alkalinized Water in Lean and Obese Zucker Rats
    Article Snippet: Tissue fixation and staining were performed using the Vectastain ABC kit and the DAB peroxidase substrate kit (both from Vector Laboratories, Burlingame, CA, USA) following the manufacturer’s instructions. .. Samples were incubated with anti-F4/80 primary antibody (MCA497; AbD Serotec, Oxford, UK) and then with anti-rat secondary antibody (NA935 GE Healthcare, Boston, MA, USA) linked to alkaline phosphatase (AP), exposed to the AP substrate and, once staining developed, mounted. .. Images were acquired with a Nikon E90i microscope equipped with a DS-Fi1 camera (Nikon, Tokyo, Japan).

    Article Title: Pro-inflammatory macrophages impair skeletal muscle regeneration in ischemic-damaged limbs by inducing precocious differentiation of satellite cells
    Article Snippet: Cells were blocked with purified anti-mouse CD16/32 antibody (Biolegend, #101301) for 10 minutes. .. Primary antibody staining was performed using anti-CD45-Alexa Fluor 488 (clone HI30, Invitrogen, MHCD4520), anti-CD11b (clone M1/70, Invitrogen, 12-0112-81), and anti-F4/80-biotin (clone A3-1, Bio-rad, MCA497BT) antibodies for 40 minutes. .. Streptavidin-PE/Cy7 (Biolegend, 405206) was used as a secondary reagent for anti-F4/80-biotin (PMID: 25896247).

    Article Title: Skeletal muscle regeneration failure in ischemic-damaged limbs is associated with pro-inflammatory macrophages and premature differentiation of satellite cells
    Article Snippet: .. Cells were blocked with purified anti-mouse CD16/32 antibody (Biolegend, #101301) for 10 min. Primary antibody staining was performed using anti-CD45-Alexa Fluor 488 (clone HI30, Invitrogen, MHCD4520), anti-CD11b (clone M1/70, Invitrogen, 12-0112-81), and anti-F4/80-biotin (clone A3-1, Bio-rad, MCA497BT) antibodies for 40 min. Streptavidin-PE/Cy7 (Biolegend, 405206) was used as a secondary reagent for anti-F4/80-biotin (PMID: 25896247). ..

    Article Title: Dysfunctional CD4 T cells in an oncovirus-specific TCR-transgenic in vivo model
    Article Snippet: .. Cells were stained with anti-CD11c microbeads (Miltenyi Biotec, Germany; Catalog, 130-125-835), run on an AutoMACS (Miltenyi Biotec, Germany) using the “Possel_S” program, blocked with anti-FcR mAb 2.4G2 supernatant including 5 % rat serum and stained with anti-CD11c-PE (clone HL3; BD Biosciences, USA; Catalog, 561044), anti-F4/80-FITC (clone CI:A3-1; Bio-Rad, USA; Catalog, MCA497FA) and propidium iodide (PI). .. Thymic epithelial cells were enriched by pooling cells from collagenase/neutral protease digestion rounds two to five, followed by staining with anti-CD45 microbeads (Miltenyi Biotec, Germany; Catalog, 130-052-301), run on an AutoMACS using the ‘Deplete’ program, blocked with anti-FcR mAb 2.4G2 supernatant and stained with anti-Ly51-FITC (clone 6C3; BD Biosciences, USA; Catalog, 562057), anti-CD80-PE (clone 16-10A1; BD Biosciences, USA; Catalog, 561955), anti-EpCAM-Alexa647 (clone G8.8; kindly provided by Dr. B. Kyewski), anti-CD45-PE/Cy5 (clone 30-F11; BD Biosciences, USA; Catalog, 561870) and PI.

    Article Title: Dysfunctional CD4 T cells in an oncovirus-specific TCR-transgenic in vivo model.
    Article Snippet: .. Cells were stained with anti-CD11c microbeads (Miltenyi Biotec, Germany; Catalog, 130-125-835), run on an AutoMACS (Miltenyi Biotec, Germany) using the “Possel_S” program, blocked with anti-FcR mAb 2.4G2 supernatant including 5 % rat serum and stained with antiCD11c-PE (clone HL3; BD Biosciences, USA; Catalog, 561044), anti-F4/80-FITC (clone CI:A3-1; Bio-Rad, USA; Catalog, MCA497FA) and propidium iodide (PI). .. Thymic epithelial cells were enriched by pooling cells from collagenase/neutral protease digestion rounds two to five, followed by staining with anti-CD45 microbeads (Miltenyi Biotec, Germany; Catalog, 130-052-301), run on an AutoMACS using the ‘Deplete’ program, blocked with anti-FcR mAb 2.4G2 supernatant and stained with anti-Ly51-FITC (clone 6C3; BD Biosciences, USA; Catalog, 562057), anti-CD80-PE (clone 16-10A1; BD Biosciences, USA; Catalog, 561955), anti-EpCAM-Alexa647 (clone G8.8; kindly provided by Dr. B. Kyewski), anti-CD45-PE/Cy5 (clone 30-F11; BD Biosciences, USA; Catalog, 561870) and PI.

    Article Title: Macrophages restrict tumor permissiveness to immune infiltration by controlling local collagen topography through a Tcf4-Collagen3 fibrotic axis
    Article Snippet: Sections were permeabilized using 0,2% Triton (Sigma, T8787) diluted PBS, blocked using 1:100 Fcblock (BD, 553142, 1:100), 1:20 Goat serum (JacksonImmunoResearch, 005-000-121) or Donkey serum (JacksonImmunoResearch, 0017-000-121) depending on the host of secondary antibodies, and 3% IgG-free Bovine Serum Albumin (JacksonImmunoResearch,001-000-162) in 0,05% Triton in PBS. .. They were stained with the following primary antibodies: anti-F4/80-AF647 (Biorad, MCA497A647, 1:100), anti-CD3e (Biolegend, 100306, 1:200), anti-Ly6G (Biolegend, 127605, 1:200), anti-Col3a1 (SouthernBiotech,1330-01, 1:300), anti-Tcf4 (ProteinTech, 16801875, 1:100). .. For Col3a1 and Tcf4 stainings, donkey anti-goat AF647 (Invitrogen, A21202, 1:200) and donkey anti-rabbit AF546 (Invitrogen, A10040, 1:00) secondary antibodies were used respectively.

    Purification:

    Article Title: Skeletal muscle regeneration failure in ischemic-damaged limbs is associated with pro-inflammatory macrophages and premature differentiation of satellite cells
    Article Snippet: .. Cells were blocked with purified anti-mouse CD16/32 antibody (Biolegend, #101301) for 10 min. Primary antibody staining was performed using anti-CD45-Alexa Fluor 488 (clone HI30, Invitrogen, MHCD4520), anti-CD11b (clone M1/70, Invitrogen, 12-0112-81), and anti-F4/80-biotin (clone A3-1, Bio-rad, MCA497BT) antibodies for 40 min. Streptavidin-PE/Cy7 (Biolegend, 405206) was used as a secondary reagent for anti-F4/80-biotin (PMID: 25896247). ..



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    Prolonged inflammatory cell accumulation in Ext1‐deficient mice during lipopolysaccharide (LPS)‐induced lung injury. (A) Immunohistochemical staining for CD11b + cells at 0–48 h after LPS administration. (B) Quantification of CD11b + cells. Ext1‐deficient mice (red line) show higher CD11b + cell retention at 48 h, indicating delayed inflammatory resolution. (C) Immunohistochemical staining <t>for</t> <t>F4/80</t> + macrophages. (D) Quantification of F4/80 + cells. Cre + Ext1 flox/flox mice show persistent and increased macrophage accumulation, consistent with impaired inflammatory clearance. Data represent mean ± standard error of the mean (SEM). * p < 0.05 vs. Cre − controls.
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    Image Search Results


    Prolonged inflammatory cell accumulation in Ext1‐deficient mice during lipopolysaccharide (LPS)‐induced lung injury. (A) Immunohistochemical staining for CD11b + cells at 0–48 h after LPS administration. (B) Quantification of CD11b + cells. Ext1‐deficient mice (red line) show higher CD11b + cell retention at 48 h, indicating delayed inflammatory resolution. (C) Immunohistochemical staining for F4/80 + macrophages. (D) Quantification of F4/80 + cells. Cre + Ext1 flox/flox mice show persistent and increased macrophage accumulation, consistent with impaired inflammatory clearance. Data represent mean ± standard error of the mean (SEM). * p < 0.05 vs. Cre − controls.

    Journal: The FASEB Journal

    Article Title: Endothelial Exostosin‐1 Is Needed for Glycocalyx Integrity and Inflammatory Resolution in Acute Lung Injury

    doi: 10.1096/fj.202601250R

    Figure Lengend Snippet: Prolonged inflammatory cell accumulation in Ext1‐deficient mice during lipopolysaccharide (LPS)‐induced lung injury. (A) Immunohistochemical staining for CD11b + cells at 0–48 h after LPS administration. (B) Quantification of CD11b + cells. Ext1‐deficient mice (red line) show higher CD11b + cell retention at 48 h, indicating delayed inflammatory resolution. (C) Immunohistochemical staining for F4/80 + macrophages. (D) Quantification of F4/80 + cells. Cre + Ext1 flox/flox mice show persistent and increased macrophage accumulation, consistent with impaired inflammatory clearance. Data represent mean ± standard error of the mean (SEM). * p < 0.05 vs. Cre − controls.

    Article Snippet: Sections were incubated with primary antibodies against CD11b (Abcam, ab16667; 1:200), F4/80 (Bioss, bs‐10701R; 1:200), or heparan sulfate (10E4 clone; Amsbio, #370255; 1:100) overnight at 4°C.

    Techniques: Immunohistochemical staining, Staining